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Human Extracellular sulfatase Sulf-2 (SULF2) ELISA Kit

Human Extracellular sulfatase Sulf-2 (SULF2) ELISA Kit

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Specification

Product name Human Extracellular sulfatase Sulf-2 (SULF2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Extracellular sulfatase Sulf-2 (SULF2) ELISA Kit employs a two-site sandwich ELISA to quantitate SULF2 in samples. An antibody specific for SULF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySULF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SULF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SULF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SULF2; RP5-1049G16.1; DKFZp313E091; FLJ44488; FLJ90554; HSULF-2; KIAA1247; MGC126411; extracellular sulfatase SULF-2

Product Properties

Kit components • Human Extracellular sulfatase Sulf-2 microplate
• Human Extracellular sulfatase Sulf-2 standard
• Human Extracellular sulfatase Sulf-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Extracellular sulfatase Sulf-2 (SULF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SULF2. No significant cross-reactivity or interference between Human SULF2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Sulfatase 2contains an N-terminal signal sequence, followed by a sulfatase domain, a hydrophilic region, and a C-terminal substrate recognition domain.SULF2 shares about 64% identity with SULF1 and 94% identity with mouse Sulf2. PCR detected SULF2 expression in most human tissues examined, with highest levels in ovary, skeletal muscle, stomach, brain, uterus, heart, kidney, and placenta. Western blot analysis of transfected Chinese hamster ovary (CHO) cells and culture medium detected SULF2 proteins with apparent molecular masses of 132, 64, and 60 kD in the culture medium. Treatment with furin inhibitors resulted in a single 132-kD species, and treatment with N-glycanase reduced the molecular mass from 132 kD to 100 kD.
Gene ID 55959
Alternative SULF2; RP5-1049G16.1; DKFZp313E091; FLJ44488; FLJ90554; HSULF-2; KIAA1247; MGC126411; extracellular sulfatase SULF-2
Accession Q8IWU5

Image & description

Fig.1. Human Extracellular sulfatase Sulf-2 (SULF2) Standard Curve.

Fig.1. Human Extracellular sulfatase Sulf-2 (SULF2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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