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Human Exportin-1 (XPO1) ELISA Kit

Human Exportin-1 (XPO1) ELISA Kit

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Specification

Product name Human Exportin-1 (XPO1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Exportin-1 (XPO1) ELISA Kit employs a two-site sandwich ELISA to quantitate XPO1 in samples. An antibody specific for XPO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXPO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XPO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XPO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative XPO1; CRM1; DKFZp686B1823; CRM1; yeast; homolog; Exportin-1 (required for chromosome region maintenance); OTTHUMP00000201471; OTTHUMP00000201493; OTTHUMP00000201494; exportin 1; exportin 1 (CRM1; yeast; homolog

Product Properties

Kit components • Human Exportin-1 microplate
• Human Exportin-1 standard
• Human Exportin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Exportin-1 (XPO1) ELISA Kit has high sensitivity and excellent specificity for detection of Human XPO1. No significant cross-reactivity or interference between Human XPO1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Exportin-1 mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1.The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus.
Gene ID 7514
Alternative XPO1; CRM1; DKFZp686B1823; CRM1; yeast; homolog; Exportin-1 (required for chromosome region maintenance); OTTHUMP00000201471; OTTHUMP00000201493; OTTHUMP00000201494; exportin 1; exportin 1 (CRM1; yeast; homolog
Accession O14980

Image & description

Fig.1. Human Exportin-1 (XPO1) Standard Curve.

Fig.1. Human Exportin-1 (XPO1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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