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Human EGF protein

Human EGF protein

Views(6) Publications(0) Catalog no(PRP1956)
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Specification

Product name Human EGF protein
Activity Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.004 ng/mL. The specific activity of recombinant Human EGF is approximately >2.5 x 108IU/mg.
Preparation method E. coli
Purity > 96% as determined by SDS-PAGE.
Molecular Formula The protein has a calculated MW of 6.35 kDa.
Alternative beta-urogastrone,EGF,epidermal growth factor, hEGF, HOMG4, URG, Urogastrone

Product Properties

Formulation The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight The protein has a calculated MW of 6.35 kDa.
Usage notes Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Storage instructions Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution,  store at 2°C to 8°C for up to 1 week.  Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose  solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Epidermal growth factor (EGF) is a small growth factor containing 53 amino acid residues that promotes the proliferation of mesenchymal and epidermal cells. Mature proteins, much smaller than 53aa, are produced by protein hydrolysis in the proximal EGF domain across the membrane. EGF is well preserved in mammals, and the EGF in mature humans is 70% the same as that in mice and mice. EGF and fibroblast growth factor 2 (fgf-2) induce the proliferation of neural precursor cells isolated from specific parts of the embryonic and adult brain. EGF and somatopodin c supplemental medium were substituted for 5% thrombocytopenic anemia plasma (PPP) and had the ability to inhibit BALB/C-3T3 cell density. The biological activities of EGF include epithelium formation, angiogenesis, inhibition of gastric acid secretion, proliferation of fibroblasts, and colony formation of epidermal cells in culture.
Gene ID 1950
Alternative beta-urogastrone,EGF,epidermal growth factor, hEGF, HOMG4, URG, Urogastrone

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