Product Information

• Product name

  c-Myc (phospho Thr58) Polyclonal Antibody

• Immunogen

  Synthesized peptide derived from human c-Myc around the phosphorylation site of T58

• Host

  Rabbit

• Reactivity

  Human, Mouse, Rat

• Applications

  ELISA, IHC-P, IP, WB

• Application notes

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IP (2-5 ug/mg lysate), ELISA (1:10000). Not yet tested in other applications.

• Clonality

  Polyclonal

• Isotype

  Rabbit IgG

• Purification

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Fig.1. Western Blot analysis of various cells using Phospho-c-Myc (T58) Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of 293 cells using Phospho-c-Myc (T58) Polyclonal Antibody diluted at 1:500.

Product Properties

• Formulation

  Liquid solution

• Concentration

  1 mg/ml

• Storage buffer

  PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.

• Storage instructions

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  V-myc avian myelocytomatosis viral oncogene homolog encoded by MYC is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of MYC.

• Gene ID

  4609

• Alternative names

  MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64

• Others

  Phospho-c-Myc (T58) Polyclonal Antibody detects endogenous levels of c-Myc protein only when phosphorylated at T58.

• Accession

  P01106