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Human E3 ubiquitin-protein ligase parkin (PARK2) ELISA Kit

Human E3 ubiquitin-protein ligase parkin (PARK2) ELISA Kit

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Specification

Product name Human E3 ubiquitin-protein ligase parkin (PARK2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human E3 ubiquitin-protein ligase parkin (PARK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PARK2 in samples. An antibody specific for PARK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PARK2; AR-JP; LPRS2; PDJ; PRKN; E3 ubiquitin ligase; parkin; parkin 2

Product Properties

Kit components • Human E3 ubiquitin-protein ligase parkin microplate
• Human E3 ubiquitin-protein ligase parkin standard
• Human E3 ubiquitin-protein ligase parkin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human E3 ubiquitin-protein ligase parkin (PARK2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PARK2. No significant cross-reactivity or interference between Human PARK2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Parkin is a proteinencoded by the PARK2 gene.The precise function of this protein is unknown; however, the protein is a component of a multiprotein E3 ubiquitin ligase complex which in turn is part of the ubiquitin-proteasome system that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause a familial form of Parkinson's disease known as autosomal recessive juvenile Parkinson disease. PARK2 is the parkin gene that may cause a form of autosomal recessive juvenile Parkinson disease due to a mutation in the parkin protein. This form of genetic mutation may be one of the most common known genetic causes of early-onset Parkinson disease.
Gene ID 5071
Alternative PARK2; AR-JP; LPRS2; PDJ; PRKN; E3 ubiquitin ligase; parkin; parkin 2
Accession O60260

Image & description

Fig.1. Human E3 ubiquitin-protein ligase parkin (PARK2) Standard Curve.

Fig.1. Human E3 ubiquitin-protein ligase parkin (PARK2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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