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Human Double-strand break repair protein MRE11A (MRE11A) ELISA Kit

Human Double-strand break repair protein MRE11A (MRE11A) ELISA Kit

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Specification

Product name Human Double-strand break repair protein MRE11A (MRE11A) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Double-strand break repair protein MRE11A (MRE11A) ELISA Kit employs a two-site sandwich ELISA to quantitate MRE11A in samples. An antibody specific for MRE11A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRE11A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRE11A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRE11A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MRE11A; ATLD; HNGS1; MRE11; MRE11B; AT-like disease; DNA recombination and repair protein; double-strand break repair protein MRE11A; endo/exonuclease Mre11; meiotic recombination 11 homolog A

Product Properties

Kit components • Human Double-strand break repair protein MRE11A microplate
• Human Double-strand break repair protein MRE11A standard
• Human Double-strand break repair protein MRE11A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Double-strand break repair protein MRE11A (MRE11A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRE11A. No significant cross-reactivity or interference between Human MRE11A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MRE11A encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. MRE11A has a pseudogene on chromosome 3. Alternative splicing of MRE11A results in two transcript variants encoding different isoforms. 
Gene ID 4361
Alternative MRE11A; ATLD; HNGS1; MRE11; MRE11B; AT-like disease; DNA recombination and repair protein; double-strand break repair protein MRE11A; endo/exonuclease Mre11; meiotic recombination 11 homolog A
Accession P49959

Image & description

Fig.1. Human Double-strand break repair protein MRE11A (MRE11A) Standard Curve.

Fig.1. Human Double-strand break repair protein MRE11A (MRE11A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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