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Human DNA repair protein XRCC2 (XRCC2) ELISA Kit

Human DNA repair protein XRCC2 (XRCC2) ELISA Kit

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Specification

Product name Human DNA repair protein XRCC2 (XRCC2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human DNA repair protein XRCC2 (XRCC2) ELISA Kit employs a two-site sandwich ELISA to quantitate XRCC2 in samples. An antibody specific for XRCC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXRCC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XRCC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XRCC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative XRCC2; DKFZp781P0919; DNA repair protein XRCC2; RAD51-like; X-ray repair cross complementing protein 2; X-ray repair; complementing defective; repair in Chinese hamster

Product Properties

Kit components • Human DNA repair protein XRCC2 microplate
• Human DNA repair protein XRCC2 standard
• Human DNA repair protein XRCC2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human DNA repair protein XRCC2 (XRCC2) ELISA Kit has high sensitivity and excellent specificity for detection of Human XRCC2. No significant cross-reactivity or interference between Human XRCC2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background XRCC2participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents.Hamster cells deficient in XRCC2 showed a more than 100-fold decrease in homologous recombination induced by double-strand breaks compared with the parental cell line. This defect was corrected to almost wildtype levels by transient transfection with a plasmid expressing XRCC2. The repair defect in XRCC2 mutant cells appeared to be restricted to recombinational repair because nonhomologous end joining was normal.
Gene ID 7516
Alternative XRCC2; DKFZp781P0919; DNA repair protein XRCC2; RAD51-like; X-ray repair cross complementing protein 2; X-ray repair; complementing defective; repair in Chinese hamster
Accession O43543

Image & description

Fig.1. Human DNA repair protein XRCC2 (XRCC2) Standard Curve.

Fig.1. Human DNA repair protein XRCC2 (XRCC2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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