| Product name | Human DNA primase large subunit (PRIM2) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human DNA primase large subunit (PRIM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIM2 in samples. An antibody specific for PRIM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRIM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRIM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRIM2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | PRIM2; RP3-401D24.1; MGC75142; PRIM2A; p58; DNA primase large subunit; DNA primase polypeptide 2; DNA primase subunit p58; dJ422B11.1.1; polypeptide 2A; p58; primase polypeptide 2A; 58kDa; primase; polypeptide 2 |
| Kit components | • Human DNA primase large subunit microplate • Human DNA primase large subunit standard • Human DNA primase large subunit detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human DNA primase large subunit (PRIM2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRIM2. No significant cross-reactivity or interference between Human PRIM2 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | DNA replication in human cells is initiated by a complex apparatus containing a DNA polymerase-alpha/primase complex that is well conserved from yeast to human. The DNA polymerase-alpha/primase complex contains 4 subunits: the polymerase-alpha p180 (POLA) and p68 (POLA2) subunits, and the primase p58 (PRIM2A) and p49 (PRIM1) subunits. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. The deduced 446-amino acid protein shares 89% identity with mouse p58, with 5 regions of homology distributed over the central part of the protein. The N and C termini are less well conserved. |
| Gene ID | 5558 |
| Alternative | PRIM2; RP3-401D24.1; MGC75142; PRIM2A; p58; DNA primase large subunit; DNA primase polypeptide 2; DNA primase subunit p58; dJ422B11.1.1; polypeptide 2A; p58; primase polypeptide 2A; 58kDa; primase; polypeptide 2 |
| Accession | P49643 |
Fig.1. Human DNA primase large subunit (PRIM2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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