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Human Delta-sarcoglycan (SGCD) ELISA Kit

Human Delta-sarcoglycan (SGCD) ELISA Kit

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Specification

Product name Human Delta-sarcoglycan (SGCD) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Delta-sarcoglycan (SGCD) ELISA Kit employs a two-site sandwich ELISA to quantitate SGCD in samples. An antibody specific for SGCD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySGCD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SGCD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SGCD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SGCD; 35DAG; CMD1L; DAGD; MGC22567; SG-delta; SGCDP; SGD; 35kD dystrophin-associated glycoprotein; delta-sarcoglycan; dystrophin associated glycoprotein; delta sarcoglycan; placental delta sarcoglycan

Product Properties

Kit components • Human Delta-sarcoglycan microplate
• Human Delta-sarcoglycan standard
• Human Delta-sarcoglycan detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Delta-sarcoglycan (SGCD) ELISA Kit has high sensitivity and excellent specificity for detection of Human SGCD. No significant cross-reactivity or interference between Human SGCD and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Delta-sarcoglycan isone of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). The sarcoglycans are a family of transmembrane proteins involved in the protein complex responsible for connecting the muscle fibre cytoskeleton to the extracellular matrix, preventing damage the muscle fibre sarcolemma through shearing forces. This protein is expressed most abundantly in skeletal and cardiac muscle. The mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed.In melanocytic cells SGCD gene expression may be regulated by MITF.
Gene ID 6444
Alternative SGCD; 35DAG; CMD1L; DAGD; MGC22567; SG-delta; SGCDP; SGD; 35kD dystrophin-associated glycoprotein; delta-sarcoglycan; dystrophin associated glycoprotein; delta sarcoglycan; placental delta sarcoglycan
Accession Q92629

Image & description

Fig.1. Human Delta-sarcoglycan (SGCD) Standard Curve.

Fig.1. Human Delta-sarcoglycan (SGCD) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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