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Human Delta-aminolevulinic acid dehydratase (ALAD) ELISA Kit

Human Delta-aminolevulinic acid dehydratase (ALAD) ELISA Kit

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Specification

Product name Human Delta-aminolevulinic acid dehydratase (ALAD) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Delta-aminolevulinic acid dehydratase (ALAD) ELISA Kit employs a two-site sandwich ELISA to quantitate ALAD in samples. An antibody specific for ALAD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALAD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALAD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALAD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ALAD; RP11-10I9.1; ALADH; MGC5057; PBGS; aminolevulinate dehydratase; delta-aminolevulinic acid dehydratase; porphobilinogen synthase

Product Properties

Kit components • Human Delta-aminolevulinic acid dehydratase microplate
• Human Delta-aminolevulinic acid dehydratase standard
• Human Delta-aminolevulinic acid dehydratase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Delta-aminolevulinic acid dehydratase (ALAD) ELISA Kit has high sensitivity and excellent specificity for detection of Human ALAD. No significant cross-reactivity or interference between Human ALAD and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternatively spliced transcript variants encoding different isoforms have been identified.
Gene ID 210
Alternative ALAD; RP11-10I9.1; ALADH; MGC5057; PBGS; aminolevulinate dehydratase; delta-aminolevulinic acid dehydratase; porphobilinogen synthase
Accession P13716

Image & description

Fig.1. Human Delta-aminolevulinic acid dehydratase (ALAD) Standard Curve.

Fig.1. Human Delta-aminolevulinic acid dehydratase (ALAD) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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