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Human CXCL7 Protein, His tag (Animal-Free)

Human CXCL7 Protein, His tag (Animal-Free)

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Specification

Product name Human CXCL7 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human CXCL7 (Ala59-Asp128) (P02775) was expressed with 6×His tag at the N-terminus.
Applications His tag
Applications notes Human
Activity Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2.The ED₅₀ for this effect is <0.5 ng/mL.
Protein length The recombinant Human CXCL7 consists of 70 amino acids and predicts a molecular mass of 8.43 kDa
Preparation method E.coli
Purity > 98 % as determined by SDS-PAGE
Molecular Formula 8.43 kDa
Alternative Neutrophil Activating Protein-2, NAP-2, PBP (parent molecule), CTAP-III (precursor)

Product Properties

Formulation Lyophilized from sterile PBS,pH 7.4.
Features & Benefits <0.1 EU per μg of the protein as determined by the LAL method.
Molecular weight 8.43 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background C-X-C motif chemokine 7 (CXCL7) also named Pro-Platelet basic protein (PPBP), which is a chemokine of the intercrine alpha family. CXCL7is a 7.7 kDa protein containing 70 amino acid residues. CXCL7 is expressed by the platelets, which are activated. During vascular injury, CXCL7 controls the glucose metabolism, mitogenesis and neutrophil recruitment by the interaction with CXCR2.
Gene ID 5473
Alternative Neutrophil Activating Protein-2, NAP-2, PBP (parent molecule), CTAP-III (precursor)
Accession P02775

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