| Product name | Human CXCL10 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human CXCL10 (Val22-Pro98) (P02778) was expressed with 6×His tag at the N-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to chemoattract human PBMCs using a concentration range of 10.0 - 100.0 ng/mL. Note: Results may vary from different PBMC donors. |
| Protein length | The recombinant Human CXCL10 Protein consists of 77 amino acids and predicts a molecular mass of 9.45 kDa. |
| Preparation method | E. coli |
| Purity | > 97% as determined by SDS-PAGE. |
| Alternative | IP-10, Gamma-Interferon Inducible Protein 10, Crg-2 |
| Formulation | Lyophilized from sterile PBS, pH7.4 |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 9.45 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | The product is shipped with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | C-X-C motif chemokine 10 (CXCL10) also named Interferon gamma-induced protein 10 (IP-10), which is a chemokine of the intercrine alpha family. CXCL10 is a 8.55kDa protein containing 77 amino acid residues. CXCL10 is produced by the several cell types like monocytes and endothelial cells, which are responded for IFNγ. CXCL10 is a chemotaxis for T cells, NK cells and macrophages. CXCL10 also binds the CXCR3 that induce the cell migration and activation like T cells and dendritic cells. |
| Alternative | IP-10, Gamma-Interferon Inducible Protein 10, Crg-2 |
| Accession | P02778 |
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