| Product name | Anti-Myc Tag Mouse Monoclonal Antibody (2D5) |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Reactivity | Mammals, Bacteria |
| Applications | IF, IP, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1000), IP (1:200). |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | Myc tag; Myc Epitope; c-Myc; bHLHe39; myc proto-oncogene protein; oncogene c-Myc |
| Formulation | Liquid solution |
| Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. |
| Alternative | Myc tag; Myc Epitope; c-Myc; bHLHe39; myc proto-oncogene protein; oncogene c-Myc |
Fig.1. Immunofluorescence staining (1:2000) of Myc fusion protein in 293 cells with red and counterstained with DAPI.
Fig.2. Western blot analysis of 1ug Myc fusion protein with Anti-Myc Mouse Monoclonal Antibody (2D5) in 1:5000 dilution (lane A) and 1:10000 dilution (lane B).
Fig.3. IP (1:200)-WB (1:5000) analysis of Myc fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Myc-tag protein (lane B); IP transfected 293 cell lysate with normal Mouse IgG (lane C) or with Anti Myc tag mAb (lane D) , and without both normal Mouse IgG and Myc tag mAb (lane E).
Author：Liu, Wenjuan, et al. Publication name：Frontiers in Immunology IF：7.3
Author：Zhang Y, Zhang X X, Ye M X, et al Publication name：Cancer letters, 2018, 419: 75-83. IF：6.50
Author：Yong Zhang, XinxinZhang, et al Publication name：Cancer Letters. 2018; 419: 75-83. IF：6.50
Author：Yan, Xiaolong, et al. Publication name：Journal of Virology IF：5.4
Author：Hu C, Zhou M, Wang W, et al Publication name：Front Microbiol, 2018, 9: 9. IF：4.30
Author：Zhao C, Shen X, Wu R, et al Publication name：Sci Rep, 2017, 7(1): 12995. IF：4.29
Author：F Ma, X Du, Y Wei, Z Zhou, DZJ Clotaire Publication name：Journal of cellular physiology, 2019. IF：3.89
Author：Jinyuan Cui, Jia Hu, Zhilan Ye, et al Publication name：Science Bulletin Available online 25 May 2019 IF：3.71
Author：Fanglin Ma, Xiaomin Du, Yudong Wei, et al Publication name： J Cell Physiol. 2019 Jun;234(6):8113-8121. IF：3.7
Author：Qian G, Hu X, Li G, et al Publication name：Biochem Biophys Res Commun, 2018, 6; 498(3): 537-543. IF：3.33
Author：Yan, Peipei, et al Publication name：Journal of Assisted Reproduction and Genetics (2017): 1-11. IF：2.7
Author：Guanghui Qian, XiaohanHu, et al Publication name：Biochemical and Biophysical Research Communications. 2018; 498(3): 537-543. IF：2.69
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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