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Human Collectrin (TMEM27) ELISA Kit

Human Collectrin (TMEM27) ELISA Kit

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Product name Human Collectrin (TMEM27) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Collectrin (TMEM27) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM27 in samples. An antibody specific for TMEM27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM27 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM27 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMEM27; NX-17; NX17; 0610008J07Rik; collectrin; kidney-specific membrane protein

Product Properties

Kit components • Human Collectrin microplate
• Human Collectrin standard
• Human Collectrin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Collectrin (TMEM27) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM27. No significant cross-reactivity or interference between Human TMEM27 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TMEM27 encodes a transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. TMEM27 has an N-terminal signal sequence, followed by a noncatalytic extracellular domain, a transmembrane segment, and a cytosolic domain. The extracellular domain contains an O-glycosylation site and 2 N-glycosylation sites. Human TMEM27 shares 47.8% identity with the C-terminal end of ACE2, but it lacks an ACE2-like catalytic domain. Northern blot analysis of rat, mouse, and human tissues detected a transcript of about 1.8 kb exclusively in kidney.
Gene ID 57393
Alternative TMEM27; NX-17; NX17; 0610008J07Rik; collectrin; kidney-specific membrane protein
Accession Q9HBJ8

Image & description

Fig.1. Human Collectrin (TMEM27) Standard Curve.

Fig.1. Human Collectrin (TMEM27) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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