| Product name | Human CNTF Protein |
| Sequence | Amino acid sequence derived from Human CNTF(P26441)(Ala2-Met200) was expressed. |
| Applications | No tag |
| Applications notes | Human |
| Activity | Measured in a cell proliferation assay using TF-1. The ED50 for this effect is typically 0.35 ng/mL. The specific activity of recombinant Human CNTF is approximately >2.86 x 10 6 IU/mg. |
| Protein length | The recombinant Human CNTF consists of 199 amino acids and has a predicted molecular mass of 22 kDa. |
| Preparation method | E. coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Alternative | Ciliary Neurotrophic Factor;Ciliary Neuronotrophic Factor;HCNTF |
| Formulation | Lyophilized from sterile PBS, pH 7.5. |
| Features & Benefits | Endotoxin: < 0.1 EU per µg of the protein as determined by the LAL method. |
| Molecular weight | 22 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CNTF protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Human CNTF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. CNTF is a survival factor for various neuronal cell types. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. |
| Gene ID | 1270 |
| Alternative | Ciliary Neurotrophic Factor;Ciliary Neuronotrophic Factor;HCNTF |
| Accession | P26441 |
Fig.SDS-PAGE analysis of Human CNTF protein.
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