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Human CD274/PD-L1 protein, His tag (Animal-Free)

Human CD274/PD-L1 protein, His tag (Animal-Free)

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Specification

Product name Human CD274/PD-L1 protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from human CD274 (NP_054862.1) (Met1-Thr239)was expressed with a 6 His tag at the C-terminus.
Protein length The recombinant human CD274 consists of 227 amino acids and predicts a molecular mass of 26.8 KD. It migrates as an approximately 33-40 KD band in SDS-PAGE under reducing conditions due to glycosylation.
Preparation method HEK293 Cells
Purity >98% as determined by SDS-PAGE
Alternative Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Features & Benefits Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.
Molecular weight 26.8 KD
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD274/PD-L1 protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human CD274 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. Plays a critical role in induction and maintenance of immune tolerance to self . As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.
Gene ID 29126
Alternative Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1
Accession Q9NZQ7

Image & description

Fig. SDS-PAGE analysis of Human CD274/PD-L1 protein, His tag.

Fig. SDS-PAGE analysis of Human CD274/PD-L1 protein, His tag.

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