| Product name | Human CCL2 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human CCL2 (Gln24-lys98) (P13500) was expressed with 6×His tag at the N-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to chemoattract BaF3 cells transfected with human CCR2A.The ED₅₀ for this effect is <20 ng/mL. |
| Protein length | The recombinant Human CCL2 Protein consists of 75 amino acids and predicts a molecular mass of 8.5 kDa |
| Preparation method | E. coli |
| Purity | > 95% as determined by SDS-PAGE. |
| Alternative | Monocyte Chemotactic Protein-1,MCP-1 |
| Formulation | The protein was lyophilized from sterile PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 8.5 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | The product is shipped with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein (MCP)-1 and small inducible cytokine A2 (SCYA2)), is a small cytokine that belongs to the CC chemokine family responsible for monocyte attraction. Its cognate receptor, CCR2, play a critical role in regulating nociceptive processes during neuropathic pain. Both CCL2 and CCR2 areimplicated in induction of autoimmunity. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. Recently research also showed that CCL2 might be useful as a biomarker of fibrosis as well as a target for therapeutic intervention |
| Alternative | Monocyte Chemotactic Protein-1,MCP-1 |
| Accession | P13500.1 |
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