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Human Cardiac phospholamban (PLN) ELISA Kit

Human Cardiac phospholamban (PLN) ELISA Kit

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Product name Human Cardiac phospholamban (PLN) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Cardiac phospholamban (PLN) ELISA Kit employs a two-site sandwich ELISA to quantitate PLN in samples. An antibody specific for PLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PLN; CMD1P; PLB; cardiac phospholamban

Product Properties

Kit components • Human Cardiac phospholamban microplate
• Human Cardiac phospholamban standard
• Human Cardiac phospholamban detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Cardiac phospholamban (PLN) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLN. No significant cross-reactivity or interference between Human PLN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2 pump in cardiac muscle and skeletal muscle cells. When phospholamban is phosphorylated by protein kinase A (PKA) its ability to to inhibit the sarcoplasmic reticulum calcium pump (SERCA) is lost. Thus, activators of PKA, such as the beta-adrenergic agonist epinephrine (released by sympathetic stimulation) may enhance the rate of cardiac myocyte relaxation. Additionally, since SERCA is more active, the next action potential will cause an increased release of calcium resulting in increased contraction (positive inotropic effect). When phospholamban is not phosphorylated, such as when PKA is inactive, it can interact with and inhibit SERCA.
Gene ID 5350
Alternative PLN; CMD1P; PLB; cardiac phospholamban
Accession P26678

Image & description

Fig.1. Human Cardiac phospholamban (PLN) Standard Curve.

Fig.1. Human Cardiac phospholamban (PLN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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