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Human Calcipressin-1 (RCAN1) ELISA Kit

Human Calcipressin-1 (RCAN1) ELISA Kit

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Specification

Product name Human Calcipressin-1 (RCAN1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Calcipressin-1 (RCAN1) ELISA Kit employs a two-site sandwich ELISA to quantitate RCAN1 in samples. An antibody specific for RCAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRCAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RCAN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RCAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RCAN1; ADAPT78; CSP1; DSC1; DSCR1; MCIP1; RCN1; Down syndrome candidate region 1; Down syndrome critical region gene 1; calcipressin 1; calcium and oxidant-inducible mRNA; modulatory calcineurin-interacting pr

Product Properties

Kit components • Human Calcipressin-1 microplate
• Human Calcipressin-1 standard
• Human Calcipressin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Calcipressin-1 (RCAN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RCAN1. No significant cross-reactivity or interference between Human RCAN1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background RCAN1 interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. The predicted 171-amino acid protein contains 2 proline-rich regions, a putative DNA-binding domain, and an acidic region. Northern blot analysis revealed that the 2.2-kb DSCR1 transcript is expressed at the highest levels in fetal brain and adult heart and at lower levels in various other tissues. An additional 2-kb mRNA was detected in fetal and adult liver.
Gene ID 1827
Alternative RCAN1; ADAPT78; CSP1; DSC1; DSCR1; MCIP1; RCN1; Down syndrome candidate region 1; Down syndrome critical region gene 1; calcipressin 1; calcium and oxidant-inducible mRNA; modulatory calcineurin-interacting pr
Accession P53805

Image & description

Fig.1. Human Calcipressin-1 (RCAN1) Standard Curve.

Fig.1. Human Calcipressin-1 (RCAN1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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