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Human BMP-14 Protein, His tag (Animal-Free)

Human BMP-14 Protein, His tag (Animal-Free)

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Specification

Product name Human BMP-14 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human BMP-14 (Met381-Arg501) (P43026) was expressed with 6×His tag at the C-terminus.
Applications His tag
Applications notes Human
Activity Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <14 ng/mL.
Protein length The recombinant Human BMP-14 consists of 121amino acids and predicts a molecular mass of 14.52 kDa.
Preparation method E.coli
Purity > 98 % as determined by SDS-PAGE
Molecular Formula 14.52 kDa
Alternative bone morphogenetic protein 14, Cartilage-Derived Morphogenetic Protein-1,Growth/Differentiation Factor-5,GDF-5, CDMP-1, BMP14

Product Properties

Formulation Lyophilized from sterile 20mM sodium citrate,0.2 M NaCl, pH 3.5.
Features & Benefits <0.1 EU per μg of the protein as determined by the LAL method.
Molecular weight 14.52 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Bone Morphogenetic Protein-14 (BMP-14), known as Growth differentiation factor 5 (GDF5), is an extracellular multifunctional cytokine that is also a member of the TGFβ family. BMP-14 can bind with the TGFβ receptor and trigger SMAD protein signal transduction. BMP-14 plays a role in skeletal and joint development and increases the survival of neurons that respond to the neurotransmitter dopamine.
Gene ID 8200
Alternative bone morphogenetic protein 14, Cartilage-Derived Morphogenetic Protein-1,Growth/Differentiation Factor-5,GDF-5, CDMP-1, BMP14
Accession P43026

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