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Human Beta-type platelet-derived growth factor receptor (PDGFRB) ELISA Kit

Human Beta-type platelet-derived growth factor receptor (PDGFRB) ELISA Kit

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Specification

Product name Human Beta-type platelet-derived growth factor receptor (PDGFRB) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Beta-type platelet-derived growth factor receptor (PDGFRB) ELISA Kit employs a two-site sandwich ELISA to quantitate PDGFRB in samples. An antibody specific for PDGFRB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGFRB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFRB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFRB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PDGFRB; CD140B; JTK12; PDGFR; PDGFR1; CD140 antigen-like family member B; PDGF-R-beta; platelet-derived growth factor receptor beta

Product Properties

Kit components • Human Beta-type platelet-derived growth factor receptor microplate
• Human Beta-type platelet-derived growth factor receptor standard
• Human Beta-type platelet-derived growth factor receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Beta-type platelet-derived growth factor receptor (PDGFRB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDGFRB. No significant cross-reactivity or interference between Human PDGFRB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PDGFRb is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
Gene ID 5159
Alternative PDGFRB; CD140B; JTK12; PDGFR; PDGFR1; CD140 antigen-like family member B; PDGF-R-beta; platelet-derived growth factor receptor beta
Accession P09619

Image & description

Fig.1. Human Beta-type platelet-derived growth factor receptor (PDGFRB) Standard Curve.

Fig.1. Human Beta-type platelet-derived growth factor receptor (PDGFRB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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