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Human Beta-2 adrenergic receptor (ADRB2) ELISA Kit

Human Beta-2 adrenergic receptor (ADRB2) ELISA Kit

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Specification

Product name Human Beta-2 adrenergic receptor (ADRB2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Beta-2 adrenergic receptor (ADRB2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADRB2 in samples. An antibody specific for ADRB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADRB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADRB2; ADRB2R; ADRBR; B2AR; BAR; BETA2AR; beta-2 adrenergic receptor; beta-2 adrenoceptor; catecholamine receptor

Product Properties

Kit components • Human Beta-2 adrenergic receptor microplate
• Human Beta-2 adrenergic receptor standard
• Human Beta-2 adrenergic receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Beta-2 adrenergic receptor (ADRB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADRB2. No significant cross-reactivity or interference between Human ADRB2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ADRb2 is directly associated with one of its ultimate effectors, the class C L-type calcium channel CaV1.2. This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes.
Gene ID 154
Alternative ADRB2; ADRB2R; ADRBR; B2AR; BAR; BETA2AR; beta-2 adrenergic receptor; beta-2 adrenoceptor; catecholamine receptor
Accession P07550

Image & description

Fig.1. Human Beta-2 adrenergic receptor (ADRB2) Standard Curve.

Fig.1. Human Beta-2 adrenergic receptor (ADRB2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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