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Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) ELISA Kit

Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) ELISA Kit

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Specification

Product name Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACAP1 in samples. An antibody specific for ACAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ACAP1; CENTB1; KIAA0050; Arf GAP with coiled coil; ANK repeat and PH domains 1; centaurin beta1; centaurin; beta 1

Product Properties

Kit components • Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 microplate
• Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 standard
• Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACAP1. No significant cross-reactivity or interference between Human ACAP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ACAP1 and ACAP2 were recruited to platelet-derived growth factor (PDGF)-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. The deduced protein contains 740 amino acids and shares 52% sequence identity with CENTB2. highest expression in spleen, thymus, and peripheral blood leukocytes, intermediate expression in lung, testis, and small intestine, and weak expression in prostate, ovary, and colon. No expression was detected in heart, brain, placenta, liver, skeletal muscle, kidney, and pancreas, and no expression was detected in HeLa cells.ACAP1 shares significant similarity with CENTB2, which the authors called ACAP2, and 95% identity with mouse Acap1.
Gene ID 9744
Alternative ACAP1; CENTB1; KIAA0050; Arf GAP with coiled coil; ANK repeat and PH domains 1; centaurin beta1; centaurin; beta 1
Accession Q15027

Image & description

Fig.1. Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) Standard Curve.

Fig.1. Human Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (ACAP1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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