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Human Alpha-Actinin 1 (ACTN-1) ELISA Kit

Human Alpha-Actinin 1 (ACTN-1) ELISA Kit

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Specification

Product name Human Alpha-Actinin 1 (ACTN-1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Alpha-Actinin 1 (ACTN-1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTN1 in samples. An antibody specific for ACTN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ACTN1; FLJ40884; FLJ54432; F-actin cross-linking protein; actinin 1 smooth muscle; alpha-actinin 1

Product Properties

Kit components • Human Alpha-Actinin 1 microplate
• Human Alpha-Actinin 1 standard
• Human Alpha-Actinin 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Alpha-Actinin 1 (ACTN-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACTN1. No significant cross-reactivity or interference between Human ACTN1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Alpha-actinin-1 is a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.
Gene ID 87
Alternative ACTN1; FLJ40884; FLJ54432; F-actin cross-linking protein; actinin 1 smooth muscle; alpha-actinin 1
Accession P12814

Image & description

Fig.1. Human Alpha-Actinin 1 (ACTN-1) Standard Curve.

Fig.1. Human Alpha-Actinin 1 (ACTN-1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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