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Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) ELISA Kit

Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) ELISA Kit

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Specification

Product name Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) ELISA Kit employs a two-site sandwich ELISA to quantitate MGAT4C in samples. An antibody specific for MGAT4C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMGAT4C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MGAT4C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MGAT4C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MGAT4C; GNTIVH; HGNT-IV-H; UDP-N-acetylglucosamine:a-1;3-D-mannoside beta-1;4-N-acetylglucosaminyltransferase IV; UDP-N-acetylglucosamine:a-1;3-D-mannoside beta-1;4-N-acetylglucosaminyltransferase IV-homolog

Product Properties

Kit components • Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C microplate
• Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C standard
• Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) ELISA Kit has high sensitivity and excellent specificity for detection of Human MGAT4C. No significant cross-reactivity or interference between Human MGAT4C and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MGAT4C (MGAT4 Family Member C) is a Protein Coding gene. Diseases associated with MGAT4C include Mannosidosis, Beta and Muscular Dystrophy, Congenital. Among its related pathways are Transport to the Golgi and subsequent modification and Metabolism of proteins. GO annotations related to this gene include transferase activity, transferring hexosyl groups and alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity. An important paralog of this gene is MGAT4A. Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains (By similarity). Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage (GnT-VI activity).
Gene ID 25834
Alternative MGAT4C; GNTIVH; HGNT-IV-H; UDP-N-acetylglucosamine:a-1;3-D-mannoside beta-1;4-N-acetylglucosaminyltransferase IV; UDP-N-acetylglucosamine:a-1;3-D-mannoside beta-1;4-N-acetylglucosaminyltransferase IV-homolog
Accession Q9UBM8

Image & description

Fig.1. Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) Standard Curve.

Fig.1. Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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