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Human Adenosylhomocysteinase (AHCY) ELISA Kit

Human Adenosylhomocysteinase (AHCY) ELISA Kit

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Specification

Product name Human Adenosylhomocysteinase (AHCY) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Adenosylhomocysteinase (AHCY) ELISA Kit employs a two-site sandwich ELISA to quantitate AHCY in samples. An antibody specific for AHCY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAHCY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AHCY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AHCY bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative AHCY; SAHH; S-adenosylhomocysteine hydrolase

Product Properties

Kit components • Human Adenosylhomocysteinase microplate
• Human Adenosylhomocysteinase standard
• Human Adenosylhomocysteinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Adenosylhomocysteinase (AHCY) ELISA Kit has high sensitivity and excellent specificity for detection of Human AHCY. No significant cross-reactivity or interference between Human AHCY and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. In eukaryotes, this is the major route for disposal of the S-adenosylhomocysteine formed as a common product of each of many S-adenosylmethionine-dependent methyltransferases. The reaction is reversible, but under normal conditions the removal of both adenosine and homocysteine is sufficiently rapid to maintain the flux in the direction of hydrolysis.
Gene ID 191
Alternative AHCY; SAHH; S-adenosylhomocysteine hydrolase
Accession P23526

Image & description

Fig.1. Human Adenosylhomocysteinase (AHCY) Standard Curve.

Fig.1. Human Adenosylhomocysteinase (AHCY) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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