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Human ADAMTS-like protein 2 (ADAMTSL2) ELISA Kit

Human ADAMTS-like protein 2 (ADAMTSL2) ELISA Kit

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Specification

Product name Human ADAMTS-like protein 2 (ADAMTSL2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human ADAMTS-like protein 2 (ADAMTSL2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTSL2 in samples. An antibody specific for ADAMTSL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTSL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTSL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTSL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADAMTSL2; FLJ45164; KIAA0605;

Product Properties

Kit components • Human ADAMTS-like protein 2 microplate
• Human ADAMTS-like protein 2 standard
• Human ADAMTS-like protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human ADAMTS-like protein 2 (ADAMTSL2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAMTSL2. No significant cross-reactivity or interference between Human ADAMTSL2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ADAMTSL2 encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) and ADAMTS-like protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene lacks the protease domain, and is therefore of a member of the the ADAMTS-like protein subfamily. It is a secreted glycoprotein that binds the cell surface and extracellular matrix; it also interacts with latent transforming growth factor beta binding protein 1. Mutations in this gene have been associated with geleophysic dysplasia.
Gene ID 9719
Alternative ADAMTSL2; FLJ45164; KIAA0605;
Accession Q86TH1

Image & description

Fig.1. Human ADAMTS-like protein 2 (ADAMTSL2) Standard Curve.

Fig.1. Human ADAMTS-like protein 2 (ADAMTSL2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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