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Human Activin receptor type-1 (ACVR1) ELISA Kit

Human Activin receptor type-1 (ACVR1) ELISA Kit

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Specification

Product name Human Activin receptor type-1 (ACVR1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Activin receptor type-1 (ACVR1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACVR1 in samples. An antibody specific for ACVR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACVR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACVR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACVR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ACVR1; ACTRI; ACVR1A; ACVRLK2; ALK2; FOP; SKR1; TSRI; OTTHUMP00000204604; OTTHUMP00000204626; TGF-B superfamily receptor type I; activin A receptor; type-like kinase 2; hydroxyalkyl-protein kinase; serine/th

Product Properties

Kit components • Human Activin receptor type-1 microplate
• Human Activin receptor type-1 standard
• Human Activin receptor type-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Activin receptor type-1 (ACVR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACVR1. No significant cross-reactivity or interference between Human ACVR1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors.
Gene ID 90
Alternative ACVR1; ACTRI; ACVR1A; ACVRLK2; ALK2; FOP; SKR1; TSRI; OTTHUMP00000204604; OTTHUMP00000204626; TGF-B superfamily receptor type I; activin A receptor; type-like kinase 2; hydroxyalkyl-protein kinase; serine/th
Accession Q04771

Image & description

Fig.1. Human Activin receptor type-1 (ACVR1) Standard Curve.

Fig.1. Human Activin receptor type-1 (ACVR1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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