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Human Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit

Human Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit

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Specification

Product name Human Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit employs a two-site sandwich ELISA to quantitate ACACA in samples. An antibody specific for ACACA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACACA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACACA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACACA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ACACA; ACAC; ACC; ACC1; ACCA; ACC-alpha; acetyl-CoA carboxylase 1; acetyl-CoA carboxylase-alpha

Product Properties

Kit components • Human Acetyl-CoA carboxylase 1 microplate
• Human Acetyl-CoA carboxylase 1 standard
• Human Acetyl-CoA carboxylase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Acetyl-CoA carboxylase 1 (ACACA) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACACA. No significant cross-reactivity or interference between Human ACACA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes the irreversible carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes and in the chloroplasts of most plants and algae, whereas it is a large, multi-domain enzyme in the endoplasmic reticulum of most eukaryotes. The most important function of ACC is to provide the malonyl-CoA substrate for the biosynthesis of fatty acids. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs — ACACA and ACACB. In muscle cells, malonyl-CoA inhibits beta-oxidation.
Gene ID 31
Alternative ACACA; ACAC; ACC; ACC1; ACCA; ACC-alpha; acetyl-CoA carboxylase 1; acetyl-CoA carboxylase-alpha
Accession Q13085

Image & description

Fig.1. Human Acetyl-CoA carboxylase 1 (ACACA) Standard Curve.

Fig.1. Human Acetyl-CoA carboxylase 1 (ACACA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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