| Product name | Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACAT2 in samples. An antibody specific for ACAT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACAT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACAT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACAT2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | ACAT2; Acetoacetyl Coenzyme A thiolase; cytosolic acetoacetyl-CoA thiolase |
| Kit components | • Human Acetyl-CoA acetyltransferase, cytosolic microplate • Human Acetyl-CoA acetyltransferase, cytosolic standard • Human Acetyl-CoA acetyltransferase, cytosolic detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACAT2. No significant cross-reactivity or interference between Human ACAT2 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ACAT2 is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.As the human TCP1 gene had been assigned to 6q25-q27 by study of somatic cell hybrids and by in situ hybridization, the ACAT2 gene was suspected to be localized to the same chromosome region. Retention of this close linkage during mammalian evolution suggests the possibility of some functional significance. Transcription of both DNA strands at a given locus is common in prokaryotic and viral systems. For examples of overlapping transcriptional units in humans. |
| Gene ID | 39 |
| Alternative | ACAT2; Acetoacetyl Coenzyme A thiolase; cytosolic acetoacetyl-CoA thiolase |
| Accession | Q9BWD1 |
Fig.1. Human Acetyl-CoA acetyltransferase, cytosolic (ACAT2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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