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Human Abl interactor 1 (ABI1) ELISA Kit

Human Abl interactor 1 (ABI1) ELISA Kit

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Specification

Product name Human Abl interactor 1 (ABI1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Abl interactor 1 (ABI1) ELISA Kit employs a two-site sandwich ELISA to quantitate ABI1 in samples. An antibody specific for ABI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ABI1; RP13-16H11.4; ABI-1; E3B1; NAP1BP; SSH3BP; SSH3BP1; Abelson interactor 1; Abl-interactor protein 1 long; eps8 binding protein; interactor protein AblBP4; nap1 binding protein; spectrin SH3 domain binding

Product Properties

Kit components • Human Abl interactor 1 microplate
• Human Abl interactor 1 standard
• Human Abl interactor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Abl interactor 1 (ABI1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABI1. No significant cross-reactivity or interference between Human ABI1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, the encoded protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling. Abi1 is adoptor protein. It interacts with c-Abl and WAVE2 which is actin polymerization regulator. It is known that Abi1 enhances the phosphorylation of WAVE2 by c-Abl. The phosphorylation of c-Abl promotes actin polymerization.Furthermore, Abi1 is component of WAVE complex. Some research paper showed that knock down of Abi1 by si(short interffering)RNA promoted degradation of WAVE complex proteins.
Gene ID 10006
Alternative ABI1; RP13-16H11.4; ABI-1; E3B1; NAP1BP; SSH3BP; SSH3BP1; Abelson interactor 1; Abl-interactor protein 1 long; eps8 binding protein; interactor protein AblBP4; nap1 binding protein; spectrin SH3 domain binding
Accession Q8IZP0

Image & description

Fig.1. Human Abl interactor 1 (ABI1) Standard Curve.

Fig.1. Human Abl interactor 1 (ABI1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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