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Human 4F2 cell-surface antigen heavy chain (SLC3A2) ELISA Kit

Human 4F2 cell-surface antigen heavy chain (SLC3A2) ELISA Kit

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Specification

Product name Human 4F2 cell-surface antigen heavy chain (SLC3A2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human 4F2 cell-surface antigen heavy chain (SLC3A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC3A2 in samples. An antibody specific for SLC3A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC3A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC3A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC3A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SLC3A2; 4F2; 4F2HC; 4T2HC; CD98; CD98HC; MDU1; NACAE; 4F2 cell-surface antigen heavy chain; 4F2 heavy chain; CD98 heavy chain; antigen defined by monoclonal antibody 4F2; heavy chain; antigen identified by mono

Product Properties

Kit components • Human 4F2 cell-surface antigen heavy chain microplate
• Human 4F2 cell-surface antigen heavy chain standard
• Human 4F2 cell-surface antigen heavy chain detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human 4F2 cell-surface antigen heavy chain (SLC3A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC3A2. No significant cross-reactivity or interference between Human SLC3A2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SLC3A2 is a member of the solute carrier family and encodes a cell surface, transmembrane protein with an alpha-amylase domain. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. It associates with integrins and mediates integrin-dependent signaling related to normal cell growth and tumorigenesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid- trimester stage.
Gene ID 6520
Alternative SLC3A2; 4F2; 4F2HC; 4T2HC; CD98; CD98HC; MDU1; NACAE; 4F2 cell-surface antigen heavy chain; 4F2 heavy chain; CD98 heavy chain; antigen defined by monoclonal antibody 4F2; heavy chain; antigen identified by mono
Accession P08195

Image & description

Fig.1. Human 4F2 cell-surface antigen heavy chain (SLC3A2) Standard Curve.

Fig.1. Human 4F2 cell-surface antigen heavy chain (SLC3A2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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