| Product name | Human 28S ribosomal protein S28, mitochondrial (MRPS28) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human 28S ribosomal protein S28, mitochondrial (MRPS28) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS28 in samples. An antibody specific for MRPS28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPS28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS28 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | MRPS28; FLJ22853; HSPC007; MRP-S28; MRP-S35; MRPS35; mitochondrial 28S ribosomal protein S35 |
| Kit components | • Human 28S ribosomal protein S28, mitochondrial microplate • Human 28S ribosomal protein S28, mitochondrial standard • Human 28S ribosomal protein S28, mitochondrial detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human 28S ribosomal protein S28, mitochondrial (MRPS28) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRPS28. No significant cross-reactivity or interference between Human MRPS28 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS28 encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. |
| Gene ID | 28957 |
| Alternative | MRPS28; FLJ22853; HSPC007; MRP-S28; MRP-S35; MRPS35; mitochondrial 28S ribosomal protein S35 |
| Accession | Q9Y2Q9 |
Fig.1. Human 28S ribosomal protein S28, mitochondrial (MRPS28) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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