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HSP70 (Acetyl Lys246) Polyclonal Antibody

HSP70 (Acetyl Lys246) Polyclonal Antibody

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Specification

Product name HSP70 (Acetyl Lys246) Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human HSP70 around the acetylation site of K246
Host Rabbit
Reactivity Human,Rat,Mouse,
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative HSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 70kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background HSPA1A encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, heat shock protein family A (Hsp70) member 1A stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. HSPA1A is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Gene ID 3303/3304
Alternative HSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2
Others Acetyl-HSP70 (K246) Polyclonal Antibody detects endogenous levels of HSP70 protein only when non-acetylation at K246.
Accession P08107
Observed Band(KD) 70

Image & description

Fig.1. Western Blot analysis of rat kidney cells using Acetyl-HSP70 (K246) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary Antibody  was diluted at 1:20000.

Fig.1. Western Blot analysis of rat kidney cells using Acetyl-HSP70 (K246) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary Antibody was diluted at 1:20000.

Fig.2. Western Blot analysis of RAT-kidney cells using Acetyl-HSP70 (K246) Polyclonal Antibody diluted at 1:1000. Secondary Antibody  was diluted at 1:20000.

Fig.2. Western Blot analysis of RAT-kidney cells using Acetyl-HSP70 (K246) Polyclonal Antibody diluted at 1:1000. Secondary Antibody was diluted at 1:20000.

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