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HSP60 Polyclonal Antibody

HSP60 Polyclonal Antibody

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Specification

Product name HSP60 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human HSP60 at AA range: 480-560
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative HSPD1; HSP60; 60 kDa heat shock protein; mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 68 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background HSPD1 encodes a member of the chaperonin family. 60 kDa heat shock protein mitochondrial may function as a signaling molecule in the innate immune system. 60 kDa heat shock protein mitochondrial is essential for the folding and assembly of newly imported proteins in the mitochondria. HSPD1 is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
Gene ID 3329
Alternative HSPD1; HSP60; 60 kDa heat shock protein; mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein
Others HSP60 Polyclonal Antibody detects endogenous levels of HSP60 protein.
Accession P10809

Image & description

Fig.1. Western Blot analysis of various cells using HSP60 Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of various cells using HSP60 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of COLO205 cells using HSP60 Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of COLO205 cells using HSP60 Polyclonal Antibody diluted at 1:2000.

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