| Product name | HP-1γ Mouse Monoclonal Antibody (3B9) |
| Immunogen | Recombinant Protein of HP-1γ |
| Host | Mouse |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:50-300;IF 1:50-200 |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Chromobox protein homolog 3; HECH; Heterochromatin protein 1 homolog gamma; HP1 gamma; Modifier 2 protein |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 24kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by CBX3 (chromobox 3) binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for CBX3. |
| Gene ID | 11335 |
| Alternative | Chromobox protein homolog 3; HECH; Heterochromatin protein 1 homolog gamma; HP1 gamma; Modifier 2 protein |
| Others | The antibody detects endogenous HP-1γ protein. |
| Accession | Q13185 |
| Observed Band(KD) | 24 |
Fig.1. Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1γ Mouse mAb diluted at 1:200.
Fig.2. Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1γ Mouse mAb diluted at 1:200.
Fig.3. Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1γ Mouse mAb diluted at 1:1000.
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