| Product name | HMG-1 (Acetyl Lys12) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human HMG-1 around the acetylation site of K12 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Alternative | HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | HMGB1 encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. High mobility group box 1 plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of HMGB1 have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. |
| Gene ID | 3146 |
| Alternative | HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1 |
| Others | Acetyl-HMG-1 (K12) Polyclonal Antibody detects endogenous levels of HMG-1 protein only when non-acetylation at K12. |
| Accession | P09429 |
| Observed Band(KD) | 25 |
Fig.1. Western Blot analysis of NIH-3T3, HepG2-UV cells using Acetyl-HMG-1 (K12) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary Antibody was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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