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Histone H1 Polyclonal Antibody

Histone H1 Polyclonal Antibody

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Specification

Product name Histone H1 Polyclonal Antibody
Immunogen Synthesized peptide derived from human Histone H1 around the non-phosphorylation site of T17
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative HIST1H1B; H1F5; Histone H1.5; Histone H1a; Histone H1b; Histone H1s-3; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. HIST1H1B is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. HIST1H1B is found in the small histone gene cluster on chromosome 6p22-p21.3.
Gene ID 3009/3007/3008
Alternative HIST1H1B; H1F5; Histone H1.5; Histone H1a; Histone H1b; Histone H1s-3; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4
Others Histone H1 Polyclonal Antibody detects endogenous levels of Histone H1 protein.
Accession P16401/P16402/P10412

Image & description

Fig.1. Western Blot analysis of various cells using Histone H1 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using Histone H1 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 3T3 cells using Histone H1 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 3T3 cells using Histone H1 Polyclonal Antibody diluted at 1:1000.

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