| Product name | His-tag Polyclonal Antibody |
| Immunogen | 6X His synthetic peptide conjugated to KLH |
| Host | Rabbit |
| Reactivity | Species independent |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-1:3000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 15kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins. |
| Alternative | His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag |
| Others | His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. |
| Observed Band(KD) | 15 |
Fig.1. Western Blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2) . Antibody was diluted at 1:2000. Secondary Antibody was diluted at 1:20000.
Fig.2. Western Blot analysis of HIS-protein cells using His-tag Polyclonal Antibody diluted at 1:2000. Secondary Antibody was diluted at 1:20000.
Author：Chang J, Liu X, Ren H, Lu S, Li M, Zhang S, Zhao K, Li H, Zhou X, Peng L, Liu Z, Hu P Publication name：Mol Pharm IF：4.321
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