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HDAC5/9 (phospho Ser259/220) Polyclonal Antibody

HDAC5/9 (phospho Ser259/220) Polyclonal Antibody

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Specification

Product name HDAC5/9 (phospho Ser259/220) Polyclonal Antibody
Immunogen Synthesized peptide derived from human HDAC5/9 around the phosphorylation site of S259/220
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative HDAC5; KIAA0600; Histone deacetylase 5; HD5; Antigen NY-CO-9; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR; Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription rep

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The histone deacetylase 5 encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID 10014/9734
Alternative HDAC5; KIAA0600; Histone deacetylase 5; HD5; Antigen NY-CO-9; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR; Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription rep
Others Phospho-HDAC5/9 (S259/220) Polyclonal Antibody detects endogenous levels of HDAC5/9 protein only when phosphorylated at S259/220.
Accession Q9UQL6/Q9UKV0
Observed Band(KD) 121

Image & description

Fig.1. Western Blot analysis of HepG2 cells using Phospho-HDAC5/9 (S259/220) Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of HepG2 cells using Phospho-HDAC5/9 (S259/220) Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of COLO HELA 293T cells using Phospho-HDAC5/9 (S259/220) Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of COLO HELA 293T cells using Phospho-HDAC5/9 (S259/220) Polyclonal Antibody diluted at 1:2000.

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