| Product name | GIT2 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human GIT2 at AA range: 330-410 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | GIT2; KIAA0148; ARF GTPase-activating protein GIT2; ARF GAP GIT2; Cool-interacting tyrosine-phosphorylated protein 2; CAT-2; CAT2; G protein-coupled receptor kinase-interactor 2; GRK-interacting protein 2 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 84kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | GIT2 encodes a member of the GIT protein family, ARF GTPase-activating protein GIT2, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. GIT2 has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. GIT2 undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. |
| Gene ID | 9815 |
| Alternative | GIT2; KIAA0148; ARF GTPase-activating protein GIT2; ARF GAP GIT2; Cool-interacting tyrosine-phosphorylated protein 2; CAT-2; CAT2; G protein-coupled receptor kinase-interactor 2; GRK-interacting protein 2 |
| Others | GIT2 Polyclonal Antibody detects endogenous levels of GIT2 protein. |
| Accession | Q14161 |
| Observed Band(KD) | 84 |
Fig.1. Western Blot analysis of various cells using GIT2 Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of HepG2 cells using GIT2 Polyclonal Antibody diluted at 1:1000.
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