| Product name | GIT1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human GIT1 at AA range: 530-610 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | GIT1; ARF GTPase-activating protein GIT1; ARF GAP GIT1; Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 95kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ARF GTPase-activating protein GIT1 is an enzyme that in humans is encoded by the GIT1 gene. GIT1 contains an ARFGAP domain, Anykrin repeats and a GRK-interacting domain. The Arf-GAP domain, which enables it to act as a GTPase activating protein (GAP) for the Arf family of GTPases, has been shown to be involved in phosphorylation and inhibition of the ADRB2. If synaptic localization of GIT1 is disturbed, then this is known to affect dendritic spine morphology and formation---this is thought to occur through the Rac1/PAK1/LIMK/CFL1 pathway. |
| Gene ID | 28964 |
| Alternative | GIT1; ARF GTPase-activating protein GIT1; ARF GAP GIT1; Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1 |
| Others | GIT1 Polyclonal Antibody detects endogenous levels of GIT1 protein. |
| Accession | Q9Y2X7 |
| Observed Band(KD) | 95 |
Fig.1. Western Blot analysis of various cells using GIT1 Polyclonal Antibody.
Fig.2. Western Blot analysis of HepG2 cells using GIT1 Polyclonal Antibody.
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