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GCK Polyclonal Antibody

GCK Polyclonal Antibody

Views(19) Publications(0) Catalog no(ABP51409)
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Specification

Product name GCK Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human GCK
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative GCK; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. Glucokinase localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Gene ID 2645
Alternative GCK; Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
Others GCK Polyclonal Antibody detects endogenous levels of GCK protein.
Accession P35557
Observed Band(KD) 55

Image & description

Fig.1. Western Blot analysis of various cells using GCK Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using GCK Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of NIH-3T3 cells using GCK Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of NIH-3T3 cells using GCK Polyclonal Antibody diluted at 1:1000.

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