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GATA-4 Polyclonal Antibody

GATA-4 Polyclonal Antibody

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Product name GATA-4 Polyclonal Antibody
Immunogen Synthesized peptide derived from human GATA-4 around the non-phosphorylation site of S262
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative GATA4; Transcription factor GATA-4; GATA-binding factor 4

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background GATA4 encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants.
Gene ID 2626
Alternative GATA4; Transcription factor GATA-4; GATA-binding factor 4
Others GATA-4 Polyclonal Antibody detects endogenous levels of GATA-4 protein.
Accession P43694
Observed Band(KD) 48

Image & description

Fig. Western Blot analysis of COLO cells using GATA-4 Polyclonal Antibody.

Fig. Western Blot analysis of COLO cells using GATA-4 Polyclonal Antibody.

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