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Galectin-3 Monoclonal Antibody

Galectin-3 Monoclonal Antibody

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Specification

Product name Galectin-3 Monoclonal Antibody
Immunogen Protein
Host Mouse
Reactivity Human, Mouse
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:2000-1:5000), IHC-P (1:100-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative LGALS3; MAC2; Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background LGALS3 (galectin 3) encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.
Gene ID 3958
Alternative LGALS3; MAC2; Galectin-3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen
Accession P17931
Observed Band(KD) 26

Image & description

Fig.1. Western blot analysis of 1) MCF7, 2) 3T3 with Galectin-3 Mouse mAb diluted at 1:2000.

Fig.1. Western blot analysis of 1) MCF7, 2) 3T3 with Galectin-3 Mouse mAb diluted at 1:2000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-colon using antibody diluted at 1:50.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-colon using antibody diluted at 1:50.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-colon2 using antibody diluted at 1:50.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-colon2 using antibody diluted at 1:50.

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