Product name | FN1 Polyclonal Antibody |
Immunogen | Synthesized peptide derived from the C-terminal region of human FN1 at AA range: 2310-2390 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Applications | ELISA, IHC-P, WB |
Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications. |
Clonality | Polyclonal |
Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Alternative | FN1; FN; Fibronectin; FN; Cold-insoluble globulin; CIG |
Formulation | Liquid solution |
Concentration | 1 mg/ml |
Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | FN1 encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. |
Gene ID | 2335 |
Alternative | FN1; FN; Fibronectin; FN; Cold-insoluble globulin; CIG |
Others | FN1 Polyclonal Antibody detects endogenous levels of FN1 protein. |
Accession | P02751 |
Observed Band(KD) | 260 |
Fig.1. Western Blot analysis of various cells using FN1 Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of HeLa cells using FN1 Polyclonal Antibody diluted at 1:2000.
Fig.3. Western blot analysis of 3T3 KB K562 293T lysis using FN1 antibody. Antibody was diluted at 1:2000.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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