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FITC, Goat Anti-Rabbit IgG

Product Information

  • Product name

    FITC, Goat Anti-Rabbit IgG

  • Immunogen

    Rabbit IgG whole molecule

  • Host

    Goat

  • Reactivity

    Rabbit

  • Applications

    FCM, ICC, IF

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested 1:50-1:1000 for most fluorescent applications.

  • Clonality

    Polyclonal

  • Isotype

    Goat IgG

  • Purification

    Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE

  • Conjugate

    FITC

Product Properties

  • Formulation

    Liquid solution

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.

  • Others

    The antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

  1. Mallotus oblongifolius extracts ameliorate ischemic nerve damage by increasing endogenous neural stem cell proliferation through Wnt/β-catenin signaling pathway

    S Li, Y Song, R Deng. Food & Function, 2019

  2. Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts

    Jing Kong, Hongzhu Tian, Molecular Cancer (2019). 18:175

  3. Modulating macrophage polarization on titanium implant surface by poly(dopamine)‐assisted immobilization of IL4

    Y Wang, H Qi, RJ Miron, Y Zhang. Clinical Implant Dentistry and Related Research, 2019.

  4. Endometrial TGF-β, IL-10, IL-17 and autophagy are dysregulated in women with recurrent implantation failure with chronic endometritis

    W Wang, H Zhang, Z Chen, W Zhang. Reproductive Biology and Endocrinology, 2019.

  5. High temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis

    J Wang, WJ Gao, SL Deng. Stem Cell Research & Therapyvolume 10, Article number: 227 (2019).

  6. Anti-high mobility group box-1 antibody attenuated vascular smooth muscle cell phenotypic switching and vascular remodelling after subarachnoid haemorrhage in rats

    Liang Wang, Zhaosi Zhang, Luodan Liang, et al. Neuroscience Letters.

  7. The immune function of prophenoloxidase from red swamp crayfish (Procambarus clarkii) in response to bacterial infection

    Zhendong Qin, V. Sarath Babu, Hanzuo Lin, et al. Fish Shellfish Immunol. 2019 May 4;92:83-90.

  8. Telbivudine antagonizes TLR4 to inhibit the epithelial-to-mesenchymal transition in human proximal tubular epithelial cells in vitro

    Jie Chen, Detian Li, Enjie Luo. Int Immunopharmacol. 2019 Jun 17;74:105683.

  9. lncITPF Promotes Pulmonary Fibrosis by Targeting hnRNP-L Depending on Its Host Gene ITGBL1

    Xiaodong Song, Pan Xu, Chao Meng, et al. Mol Ther. 2019 Feb 6;27(2):380-393.

  10. 3D bioprinted glioma cell‐laden scaffolds enriching glioma stem cells via epithelial–mesenchymal transition

    Xuanzhi Wang, Xingliang Dai, Xinzhi Zhang, et al. J Biomed Mater Res A. 2019 Feb;107(2):383-391.

  11. SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells

    Xue-Qing Wan, Jia-Yu Cai, Yue Zhu, et al. International Journal of Molecular Medicine.

  12. Endometrial TGF-β, IL-10, IL-17 and autophagy are dysregulated in women with recurrentimplantation failure with chronic endometritis.

    Wen-juan Wang, Hong Zhang, Zhong-quan Chen, et al. Reprod Biol Endocrinol. 2019 Jan 3;17(1):2.

  13. Cullin1 binds and promotes NLRP3 ubiquitination to repress systematic inflammasome activation

    Pin Wan, Qi Zhang, Weiyong Liu, et al. FASEB JOURNAL, 17 Jan 2019.

  14. Telbivudine attenuates UUO-induced renal fibrosis via TGF-β/Smad and NF-κB signaling

    Chen J and Li D T International Immunopharmacology, 2018, 55: 1-8.

  15. Pathogenesis-related protein PR10 from Salix matsudanaKoidz exhibits resistance to salt stress in transgenic Arabidopsis thaliana

    Han X J, He X L, Qiu W M, et al. Environmental and Experimental Botany, 2017, 140: 74-82.

  16. Cx32 suppresses extrinsic apoptosis in human cervical cancer cells via the NF‑κB signalling pathway

    Lai Y, Fan L, Zhao Y, et al. Int J Oncol, 2017, 51(4): 1159-1168.

  17. Cullin1 represses systematic inflammasome activation by binding and catalyzing NLRP3 ubiquitination

    Wan P, Zhang Q, Liu W Y, et al. BioRxiv, 2018.

  18. Comparative analysis of two ferritin subunits from blunt snout bream (Megalobrama amblycephala): Characterization, expression, iron depriving and bacteriostatic activity

    Ding, Zhujin, et al. Fish & Shellfish Immunology (2017).

  19. PPARβ/δ, a Novel Regulator for Vascular Smooth Muscle Cells Phenotypic Modulation and Vascular Remodeling after Subarachnoid Hemorrhage in Rats.

    Zhang, Hongrong, et al. Scientific Reports 7 (2017): 45234.

  20. Bromocriptine Induces Autophagy-Dependent Cell Death in Pituitary Adenomas

    Geng, Xin, et al. World Neurosurgery 100 (2017): 407-416.

  21. Research of the fluorescence detection apparatus for nutrients

    Wang, Yu, et al. Applied Optics and Photonics China (AOPC2015). International Society for Optics and Photonics, 2015.

  22. Plexin-B1 indirectly affects glioma invasiveness and angiogenesis by regulating the RhoA/αvβ3 signaling pathway and SRPK1.

    Chang Y, Li L, Zhang L, et al. Tumor Biology, 2016: 1-12.

  23. Enhanced penetration of exogenous EPCs into brains of APP/PS1 transgenic mice.

    Yuan X, Mei B, Zhang L, et al. American journal of translational research, 2016, 8(3): 1460.

Cat #: A22120

  • Size
  • Price
  • 100μl $25
  • 500μl $100

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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