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FAK (phospho Tyr397) Polyclonal Antibody

FAK (phospho Tyr397) Polyclonal Antibody

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Specification

Product name FAK (phospho Tyr397) Polyclonal Antibody
Immunogen Synthesized peptide derived from human FAK around the phosphorylation site of Y397
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PTK2 encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Protein tyrosine kinase 2 is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of PTK2 may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for PTK2, but the full-length natures of only four of them have been determined.
Gene ID 5747
Alternative PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
Others Phospho-FAK (Y397) Polyclonal Antibody detects endogenous levels of FAK protein only when phosphorylated at Y397.
Accession Q05397
Observed Band(KD) 119

Image & description

Fig.1. Western Blot analysis of 293T(1), AD293(2), diluted at 1:1000.

Fig.1. Western Blot analysis of 293T(1), AD293(2), diluted at 1:1000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue. 1, FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min) . 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue. 1, FAK (phospho Tyr397) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min) . 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

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