ExKine™ Nuclei Extraction Kit

Product Information

  • Product name

    ExKine™ Nuclei Extraction Kit

  • Application notes

    Abbkine ExKine™ Nuclei Extraction Kit enables rapid isolation of nuclei from mammalian cultured cells and homogenate tissues. The isolated nuclei can be preserved frozen several months in the included storage buffer.

Product Properties

  • Kit components

    • Lysis Buffer (10×)
    • Assay Buffer (10×)

  • Features & Benefits

    • Rapid extraction of nuclei from mammalian cultured cells and homogenate tissues.
    • A typical isolation of nuclei with this kit can be easily done in less than one hour.

  • Usage notes

    Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.

  • Storage instructions

    Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    The preparation of an extract from nuclei is often the first step for many cell biology applications, such as in vitro apoptosis, examination of the transcriptional status of cells and a source of nuclear components (chromatin, genomic DNA, histones and nuclear RNA/RNP).

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ExKine™ Nuclei Extraction Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTP4001

  • Size
  • Price
  • 50T $40
  • 200T $120

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
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