|Product name||ExKine™ Nuclei Extraction Kit (High Purity)|
|Applications notes||Abbkine ExKine™ Nuclei Extraction Kit (High Purity) is designed for the preparation of pure nuclei and fragile nuclei from mammalian cultured cells and solid tissues. Nuclei are collected by ultracentrifugation through a sucrose cushion and are quite clean and free of contaminating membranes and cytoplasmic components. The isolated nuclei can be preserved frozen several months in the included storage buffer.|
|Kit components||• Lysis Buffer
• 1.8M Sucrose Cushion
• Sucrose Dilution
• Nuclei Storage Buffer
|Features & Benefits||• Preparation of pure nuclei and fragile nuclei from mammalian cultured cells and solid tissues.
• Protocol conducts ultracentrifugation through a dense sucrose cushion to protect nuclei and strip away membranes and cytoplasmic contaminants.
|Usage notes||Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.|
|Storage instructions||Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The preparation of an extract from nuclei is often the first step for many cell biology applications, such as in vitro apoptosis, examination of the transcriptional status of cells and a source of nuclear components (chromatin, genomic DNA, histones and nuclear RNA/RNP).|
Author：Meng, Bosu, et al. Publication name：Journal of Analytical Methods in Chemistry IF：2.6
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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