Login Register
English
0

Cart

$ 0

ExKine™ Nuclei Extraction Kit (High Purity)

ExKine™ Nuclei Extraction Kit (High Purity)

Views(1241) Publications(0) Catalog no(KTP4002)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name ExKine™ Nuclei Extraction Kit (High Purity)
Applications notes Abbkine ExKine™ Nuclei Extraction Kit (High Purity) is designed for the preparation of pure nuclei and fragile nuclei from mammalian cultured cells and solid tissues. Nuclei are collected by ultracentrifugation through a sucrose cushion and are quite clean and free of contaminating membranes and cytoplasmic components. The isolated nuclei can be preserved frozen several months in the included storage buffer.

Product Properties

Kit components • Lysis Buffer
• 1.8M Sucrose Cushion
• Sucrose Dilution
• Nuclei Storage Buffer
Features & Benefits • Preparation of pure nuclei and fragile nuclei from mammalian cultured cells and solid tissues.
• Protocol conducts ultracentrifugation through a dense sucrose cushion to protect nuclei and strip away membranes and cytoplasmic contaminants.
Usage notes Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Storage instructions Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The preparation of an extract from nuclei is often the first step for many cell biology applications, such as in vitro apoptosis, examination of the transcriptional status of cells and a source of nuclear components (chromatin, genomic DNA, histones and nuclear RNA/RNP).

Image & description

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “ExKine™ Nuclei Extraction Kit (High Purity)”